Dynamics of pathomorphological changes in the structural organization of the intervertebral disc at the end of the seventh and fourteenth day of experimental opioid exposure at the ultrastructural level

In general, the modern literature pays attention to issues of spine pathology and intervertebral discs. A significant percentage vertebral disorders - scoliosis, osteochondrosis, spinal disc herniation, etc., occur as a result exposure various factors manifest in changes The aim our work was study at ultrastructural level features pathomorphological manifestations structural components end seventh fourteenth days experimental opioid exposure. Materials methods research. objects were 32 sexually mature, white, male rats, weighing 92 103 g, aged 4.5 months. Animals injected with nalbupine intramuscularly once daily (at 10-11 a.m.) for 14 days. initial dose nalbuphine 8 mg/kg during first week, 15 second week. It created conditions chronic Before sampling, animals withdrawn from experiment using dibutyl ether. Intervertebral discs rats used material study. Ultrastructural specimens prepared according accepted methods. results As sampling after 7 we found inhomogeneous osmiophilicity compaction nucleus pulposus matrix which intensively accumulated osmiophilic grains glycogen proteoglycans, increased number collagen fibers, some them heterogeneous. also noted development moderate degenerative notochondral cells, accompanied by vacuolization cytoplasm an increase heterochromatin there. After necrotic cells pulposus, well destruction fibers annulus fibrosus found. particular, amount nucleui decrease volume swelling cytoplasm. Active fibroblasts often visualized fibrosus. Intense thickening observed areas fibrous ring. Focal noted. fibrils disintegrated into fine-grained stratified mass located loosely. Conclusions. At week that cytoplasmic processes chondrocytes decreased volume, shortened, underwent fragmentation destruction, detached surface plasmolemma. signs progressed manifested chondrocytes. Also focal